Behavioural responses of anxiety in aversive and nonaversive. Exposure to dihp, a commercial phthalate ester plasticizer used in flooring manufacturing, during the fetal period of sexual differentiation disrupts male reproductive development resulting in reproductive malformations and reduced androgendependent reproductive tissue weights in adult sd male rat offspring. To examine whether this observation may be species specific or more generalisable, the effect of this diet in rats was examined. All books are in clear copy here, and all files are secure so dont worry about it. The rats are maintained as an outbred closed colony. Sprague dawley rat detailed specifications janvier labs. Cd igs rat model information sheet charles river laboratories. Mccomb dj, kovacs k, beri j, zak f 1984 pituitary adenoma in old spraguedawley rats. Comparative analysis of growth characteristics of sprague dawley. No differences in behavior, body weight, or food consumption were observed. Sep 23, 2014 atorvastatin can downregulate the expression of receptor for advanced glycation end products rage in the aortas of diabetic rats. Hemodynamic parameters could be recorded in 85% of rats.
Using plant and animal whole proteins, 70dold female spraguedawley rats were randomised to either a normalprotein np. This study shows that physicochemical and sensory attributes of pasteurized buffalo milk fortified with ferrous sulfate 0. Modulation of n methyl n nitrosourea induced mammary. We compared the two methods in adult male sprague dawley rats. The study was conducted according to oecd guideline 407. Taconics proven process ensures timedpregnant rats bear litters within a 24hour window of your desired gestational age. Laboratory rats such as the sprague dawley sd rats are an important model for biomedical studies in relation to human physiological or pathogenic processes. Beginning at eight weeks of age and continuing daily for 21. About sprague dawley rats lauren and heathers blog. Gut microbiota dysbiosis in cafeteria diet fed sprague dawley rats article pdf available in advances in microbiology 0812. Dietary concentrations of glyphosate were adjusted weekly during growth, and between mating rest periods to achieve dose levels of 0, 3, 10, and 30 mgkgday. Serum levels of inflammatory markers il6, crp, mcp1 and tnf. Three months later, they were inoculated intranasally with h. Induction of necrotizing enterocolitis in nonpremature.
Untreated rats in the zero spent more time in open zones and exhibited more head dips than in the plus whereas start latency and closed area entries were lower in the zero than in the plus. Presented at triangle consortium for reproductive biology, durham, nc, february 23, 2008. The objectives of the present study were to evaluate the effects of a methioninesupplemented diet on systolic blood pressure bp and vasomotor functions in spraguedawley sd and deoxycorticosterone acetate docasalt hypertensive rats. Like other rat breeds used in research, the sprague dawley makes for a good general model for the study of human toxicology, reproduction, pharmacology, and. Spraguedawley and wistar rats are both established models of sucroseinduced ir and hypertg pagliassotti et al. Like other rat breeds used in research, the sprague dawley makes for a good general model for the study of human toxicology, reproduction, pharmacology, and behavioral research. Glucometabolic effects of single and repeated exposure to. Dawley in 1925, from a hooded male hybrid of unknown origin and an albino female probably wistar, and was crossed with the. The study has been extended to senescent rats to follow changes in the heart proteome possibly occurring in centenarians. Characteristics include albino coloring, docile behavior, and low fertility. Our recent study is the first study that demonstrated the presence of tyrosine phosphorylated proteins in epithelium and fluid of epididymis of spraguedawley rats. However, the safety of this consumption modality has not been vetted.
Complete 2dimensional echocardiography for cardiac anatomy and function was obtained in 44 rats. Lewis, fischer 344, and spraguedawley rats display. To identify a choline dietary concentration, sprague dawley rats 56sexgroup were fed control diet or 5x, 10x, or 15x basal choline diets for four weeks. We tested exogenous ketone supplements added to food and fed chronically for 83 days in spd rats and administered. History of the sprague dawley rat erikacarlys weblog. The lewis rat was developed by margaret lewis from wistar stock in the early 1950s. The focus of this study was to determine the effects of ketone supplementation on anxietyrelated behavior in spraguedawley spd and wistar albino glaxorijswijk wagrij rats. Diverse spectrum of tumors in male spraguedawley rats. I am a model organism used by duke university to research the impacts of chemical exposure on behavior, memory, and cognitive ability. These rats were obtained from the laboratory animal resource unit, universiti. Thirty adult male sprague dawley rats 12 weeks old were divided randomly into five groups. The overall analyses did not indicate significant effects of gmass nor hf diet relative to tnf. The aim of the study is to screen in sprague dawley rats the potential for dermal toxicity, absorption and metabolism of fluorochemicals from treated, dried test articles and also from the liquid state.
Accordingly, to investigate the clearance of tissue silver concentrations following oral silver nanoparticle exposure, spraguedawley rats were assigned to 3 groups. Sprague dawley rat an overview sciencedirect topics. Is a divergent central serotonergic activity responsible. Report of final results regarding brain and heart tumors in. In the infamous study where the genetic predisposition for cancer was called out, researchers were using the spraguedawley strain of laboratory rat. Regulation of mdrlb gene expression in fischer, wistar and. Pdf gut microbiota dysbiosis in cafeteria diet fed.
To investigate the basis of this difference we further examined the regulation of the mdrlb gene in hepatocytes isolated from fischer, sprague. The most common tumors were those of the mammary gland and of the nervous system. We will not get them until we are bereft of our current rats, and of course, we would sterilize the cage, first. The objective of this study was to evaluate the toxic characteristics of rauvolfia tetraphylla leaf extract in sprague dawley rats after treatment for minimum 7 days by oral route. A total of 44 spraguedawley male rats had baseline echocardiography replicating a protocol for clinical echocardiography. Genetic characterization of outbred sprague dawley rats. Ijerph free fulltext the accumulation and metabolism. However, you may choose to have us breed the founders further to obtain f1 rats. In 1991, eight colonies were selected to form the igs. Heterotropic splenic autotransplantation in the prevention of. Our research goal was to calculate a dose of dmba at each age in which 50% of the rats developed carcinoma in situ cis within three months ic50 in this strain of sprague dawley female rats. Research paper charles river sprague dawley rats lack. Wi han rats obtained primarily from charles river, quebec, canada used as control animals. The significance of primary tumors in the ntp study of.
Rats cd sprague dawley were administered glyphosate continuously for three successive generations. Spraguedawley rat definition of spraguedawley rat by. This section will focus on a brief summary of the light and ultrastructural morphology of peripheral blood cells, blood cell counts, and their variations as a function of age in spraguedawley rats of crl. Sedation improves early outcome in severely septic sprague. To investigate the basis of this difference we further examined the regulation of the mdrlb gene in hepatocytes isolated from fischer, spraguedawley and wistar rats. The text on page 2 report description describes how the studies listed here are displayed on the data pages that follow. Regardless of the health standard selected, taconic. Sprague dawley rat synonyms, sprague dawley rat antonyms. When your research demands versatility and consistency, turn to the sprague. We will breed up to 3 founders to wildtype rats of matching strain background and genotype their offspring to obtain f1 rats bearing the transgene. Effect of fermented oyster extract on growth promotion in sprague dawley rats article pdf available in integrative medicine research april 2020 with 34 reads how we measure reads. Comparison of historical control data in two strains of rat used in.
The rats average about 5 grams of food per day per 100 grams of body weight and about 1012 milliliters of water per 100 grams of body weight. Comparison between male and female spraguedawley rats in the. Biopersistence of silver nanoparticles in tissues from. Male and female spraguedawley rats were exposed from prenatal life until natural death to a 1. Is a divergent central serotonergic activity responsible for. Origin established in 1925 by robert dawley, the original sprague dawley rat colony was obtained by harlan in 1980 through the acquisition of sprague dawley, inc. The aim of this study was to observe the direct impact of atorvastatin on advanced glycation end products ages induced rage expression in healthy sprague dawley sd rats.
Animals from low, mid and high dose groups received leaf extract at 250, 500 and mgkgday, respectively. Sprague dawley sd rats based on the incidences of schwannomas of the heart. Pdf comparative analysis of growth characteristics of sprague. In this study, 30dayold male sprague dawley rats, were inoculated intraperitoneally with a single dose of 45, 90, and 180 mgkg of nethylnnitrosourea enu. Derived from bone marrow tissues of sd rats and have been transfected with a lentiviral construct encoding a gfp expression motif. Dawley during the 1920s by breeding wistar rats to hybrids of laboratory derived and wild stocks. Ntp historical controls report all routes and vehicles. Almost all laboratory and domestic pet rats belong to a single species, the norway rat rattus norvegicus. The rats did not exhibit any signs of either irritation or overt toxicity during either the exposure or postexposure periods. Therefore, this study aims to observe the effects of acute administrations of gh and ah at single doses of 2000 mgkg of body. Sprague dawley rat definition of sprague dawley rat by. Aug 19, 2009 after central venous access, sprague dawley rats underwent cecal ligation and intestinal puncture clip with an 18 g needle without antibiotic cover and received either saline, or an infusion of comparable volume of saline containing midazolam 0.
However, an acute study of gh and ah with doses of 2000 mgkg body weight on sprague dawley rats according to oecd guideline has not been carried out, even though these rats have been used intensively in honey studies. Here we report the first catalog of microbial genes in fecal samples from sprague dawley rats. Dawley sd rats, and to explore the accumulation characteristics of rees in tissues and organs with different doses as well as the detoxification and elimination of highdose rees. Sprague dawley rat synonyms, sprague dawley rat pronunciation, sprague dawley rat translation, english dictionary definition of sprague dawley rat. Sprague dawley rats, vaginally born and with 48 hours of maternal care, which are then fed with formula, to induce nec by hypoxia and temperaturecaused stress on them. Administration of 2acetylaminofluorene 2aaf to rats increased mdrlb expression in fischer, wistar and spraguedawley rat livers. The focus of this study was to determine the effects of ketone supplementation on anxietyrelated behavior in sprague dawley spd and wistar albino glaxorijswijk wagrij rats. Exogenous ketone supplements reduce anxietyrelated.
Nutritional ketosis has been proven effective for seizure disorders and other neurological disorders. It can be concluded that pasteurized milk can be an effective vehicle to fortify iron in combination with vitamin c without. Male spraguedawley cd rats and male wistar rats 200250g were supplied by charlesriver italy and were kept in temperature and humiditycontrolled rooms 22c, 50% with lights on from 0700 to 1900 hours with water and food available. The average litter size of the sprague dawley rat is 10. Administration of 2acetylaminofluorene 2aaf to rats increased mdrlb expression in fischer, wistar and sprague dawley rat livers. Feeding studies of radiation sterilization ready to eat foods. The sprague dawley breed of rat, like those used in the current psychology 381 class, is used widely throughout medical and psychological research. Methods in this study, aa was administered orally at an individual dose of 300 and 2000 mgkg body weight to group 1 and 2 respectively, while group 3 served as normal control. Animals a total of thirty two n32 adult male sprague dawley rats aged three months old weighing 200280g were used for this study. The parameters observed were body weight changed of rats, toxicological test to observe the effect free radicals formation in rats blood by using malondialdehyde mda and superoxide dismutase sod.
Nih received stock from sprague dawley, inc in 1945. Spraguedawley rat synonyms, spraguedawley rat pronunciation, spraguedawley rat translation, english dictionary definition of spraguedawley rat. Waxholm space atlas of the sprague dawley rat brain. Diisoheptyl phthalate dihp in utero exposure reduces. Diisoheptyl phthalate dihp in utero exposure reduces testicular testosterone t production in fetal sprague dawley sd rats. Baseline echocardiographic values for adult male rats. The effects of the oral administration of the probiotic strain lactobacillus salivarius lplmo1 are also studied in this model. Dawley from a hybrid hooded male and a female wistar rat. Comparison of the elevated plus and elevated zero mazes in. Charles river sprague dawley rats lack early agedependent.
Try out the sas sprague dawley rat with our animal model evaluation program. Guidelines for the housing of rats in scientific institutions. Each generation f0, f1, f2 consisted of 12 male and 24 female rats. However, it is recognised that currently available cages with maximum heights of around 22cm 24cm are. Comparison between male and female spraguedawley rats in the response of urinary biomarkers to injury induced by gentamicin jeancharles gautier, thierry gury, magali guffroy, regis masson, richard khanmalek, david hoffman, syril pettit, and ernie harpur. The current study aims to investigate the influence of five rare earth elements rees i. Sd and doca rats were fed a normal or a methionine 8 gkgsupplemented diet for 10 weeks. The current study aimed to investigate acute toxicity of aa in normal female sprague dawley rats. The sprague dawley rat and longevans rat were developed from wistar rats. However, its effect on healthy rats remains unclear. Nih began a closed colony with animals obtained from sprague dawley, inc.
The spraguedawley sd is an outbred rat, first generated by robert s. The limited ability of current treatments to control metastasis and the proposed antitumor properties of specific nutrients prompted us to examine the effect of a specific formulation nutrient supplement ns of lysine, proline, arginine, ascorbic acid, and green tea extract in vivo on the development of nmethylnnitrosourea mnuinduced mammary tumors in rats. Open access volumetric atlas offering comprehensive anatomical delineations of the rat brain based on structural contrast in isotropic magnetic resonance 39. History of the sprague dawley rat the sprague dawley breed of rat, like those used in the current psychology 381 class, is used widely throughout medical and psychological research. Sprague dawley rat article about sprague dawley rat by. The 5x diet was selected based on decreased body weights and body weight gains in the 10x females only and 15x groups. Sprague dawley rats were first bred by the sprague dawley farm in madison, wisconsin in 1925 through multiple crosses with a singlehooded male and six albino female rats. To address this, ovariectomized spraguedawley ovxsd rats were orally gavaged with purified blueberry polyphenols at 0 mg total polyphenolskg bwd for 90d. Sprague dawley sd rat has a wide array of historical research use including in the field. Also, huff 2002 reported that the positive ri findings for toluene were less than overwhelming, the negative ntp findings for vinylidene chloride less than adequate because the use of a maximum tolerated dose mtd had not been clearly demonstrated, and the positive ri findings for vinylidene chloride were for increases in leukemias and total malignant tumors in spraguedawley rats. Spraguedawley rat article about spraguedawley rat by. A 90 day oral toxicity study of blueberry polyphenols in. Inhalation of welding fumes reduced sperm counts and high fat. Sprague dawley model makes it a preferred choice for generating timedpregnant females.
Agarwal 3, aditya kulkarni 1,4 and ronit shirisverdlov 2. Looks like we will have the chance to adopt some young, unexperimented on, sprague dawley lab rats in the future. Cd sd br stock from charles river france, maintained under standardized conditions between 1982 and 1987. The effect of perfluorooctane sulfonate pfos and choline. Taconic received stock from the nih animal genetic resource in 1970. Atorvastatin inhibits the expression of rage induced by.
Schauss ag, somfairelle s, financsek i, glavits r, parent sc, endres jr, varga t, szucs z, clewell a. Sprague dawley rats were exposed for 1 hr to an aerosol concentration of 3. Effect of a methioninesupplemented diet on the blood. Report of final results regarding brain and heart tumors. The goal of this study was to further elucidate the effects of in utero dihp exposure. All procedures were carried out in accordance with the italian law legisla. Download sprague dawley rat taconic biosciences book pdf free download link or read online here in pdf. Effects of gelam and acacia honey acute administration on. A number of rat species are used in research, contract research organizations and in the pharmaceutical industry including the wistar, sprague dawley and fischer 344. In 1950, charles river laboratories crl acquired a breeding stock and developed a new line with better microbial status by caesarian derivation 22.
Oral toxicity of arjunolic acid on hematological, biochemical. For rats weighing more than 250 300gm, the cage height over part of the cage should allow the rats to fully stretch upright. It is characterized by its wide head, long ears, and a tail length that is always less than its body length. Request pdf metabolic differences between sprague dawley and wistar han male rats while sprague dawley sd rats are often used for nonclinical safety testing, wistar han wh rats are also. Spraguedawley sd rat mesenchymal stem cells with gfp. The rats were housed in individually ventilated temperature and humidity controlled cages strain and agematched two per cage with hardwood chip bedding. Inhibiting extracellular cathepsin d reduces hepatic steatosis in spraguedawley rats by princy khurana 1, tulasi yadati 2, sandeep goyal 1, atul dolas 1, tom houben 2, yvonne oligschlaeger 2, anil k.
Metabolic differences between sprague dawley and wistar. Sprague dawley rats were divided into three groups. A quantity of twenty rats ten males and ten females was submitted for testing, strain identified as line sprague dawley ntac. Spraguedawley rats, vaginally born and with 48 hours of maternal care, which are then fed with formula, to induce nec by hypoxia and temperaturecaused stress on them. To test this hypothesis, sprague dawley rats underwent sham operation, splenectomy, or splenectomy followed by autotransplantation of splenic fragments into the peritoneal cavity. The adult body weight is 250300g for females, and 450520g for males. Dawley used both his name and the maiden name, sprague, of his wife to create the name of the stock. Diazepam 1 mgkg exposure increased time in the open in both mazes. Thats a strain that has been shown to develop tumors at greater rates regardless of diet or living conditions source. The common domestic rat, which is found worldwide and is a destructive pest of crops and stored food and a carrier of disease. The wistar rat is currently one of the most popular rats used for laboratory research.
Inhibiting extracellular cathepsin d reduces hepatic. Wistar rats are more active than others like sprague dawley rats. Established in 1925 by robert dawley, the original sprague dawley rat colony was obtained by harlan in 1980 through the acquisition of spraguedawley, inc. Read online sprague dawley rat taconic biosciences book pdf free download link book now. Shorn rats were bred from sprague dawley rats in connecticut in 1998. Increases in nonneoplastic lesions of the heart, brain, and prostate gland male rats, and of the heart, thyroid in gland, and adrenal gland in female rats occurred with exposures to gsmmodulated rfr at 900 mhz. In 1991, 8 colonies were selected to form the igs foundation colony. Comparative analysis of growth characteristics of sprague. Sprague dawley rat taconic biosciences pdf book manual.